Estimation of Glycated Haemoglobin by Nephelometry, Ion Exchange Resin and High Performance Liquid Chromatography: A Cross-sectional Study
Published: September 1, 2020 | DOI: https://doi.org/10.7860/JCDR/2020/44408.13968
Mangalore Balakrishna Prathima
, Shridhar Reshma
, Sushith
, Prathiksha Shetty
,D'Sa Janice
, Bhuvanesh Sukhlal Kalal
, Gopal R Madan
1. Associate Professor, Department of Biochemistry, A. J. Institute of Medical Sciences and Research Centre, Mangaluru, Karnataka, India.
2. Associate Professor, Department of Biochemistry, A. J. Institute of Medical Sciences and Research Centre, Mangaluru, Karnataka, India.
3. Professor and Head, Department of Biochemistry, A. J. Institute of Medical Sciences and Research Centre, Mangaluru, Karnataka, India.
4. Postgraduate, Department of Biochemistry, A. J. Institute of Medical Sciences and Research Centre, Mangaluru, Karnataka, India.
5. Assistant Professor, Department of Biochemistry, A. J. Institute of Medical Sciences and Research Centre, Mangaluru, Karnataka, India.
6. Assistant Professor, Department of Pharmacology, A. J. Research Centre, A. J. Institute of Medical Sciences and Research Centre, Mangaluru, Karnataka, India.
7. Assistant Professor, Department of Biochemistry, A. J. Institute of Medical Sciences and Research Centre, Mangaluru, Karnataka, India
Correspondence
Dr. Bhuvanesh Sukhlal Kalal,
Assistant Professor, Department of Pharmacology, A. J. Research Centre, A. J. Institute
of Medical Sciences and Research Centre, Mangaluru-575004, Karnataka, India.
E-mail: bhuvanesh611@gmail.com
Introduction: Diabetes mellitus cases are continually rising all over the world. Glycated haemoglobin (HbA1c) used as a diagnostic test to measure long-term average glycaemic control in diabetic patients.
Aim: To assess the precision and reproducibility of the Ion Exchange Column Chromatography Resin (IECR) method and nephelometry in comparison to High-Performance Liquid Chromatography (HPLC) with respect to the estimation of HbA1c.
Materials and Methods: A cross-sectional comparative study was conducted on 50 blood samples, collected from the diabetic subjects at the Department of Biochemistry and their HbA1c values were estimated by HPLC based BioRad D-10, nephelometry and IECR techniques. HPLC was used as a gold standard method, to evaluate the sensitivity and specificity of nephelometry and IECR techniques. Data were expressed as mean±standard deviation and intraclass Correlation Coefficient and Pearson correlation were calculated.
Results: The mean age of the study subjects was 53.06±7.67 years. The mean plasma HbA1c levels were 8.16±2.9, 7.62±2.5 and 7.84±2.5 and mean estimated Average Glucose (eAG) was 187.00±84.4, 172.0±76.8, and 289.46±199.9 by HPLC, Nephelometry and IECR, respectively. Compared with HPLC, nephelometry had excellent correlation (r-value 0.925); p<0.001) and IECR (r-value 0.869; p<0.001).
Conclusion: Nephelometry and IECR both had better performance and showed a greater concordance with gold standard HPLC. Therefore, nephelometry and IECR can be used as an alternative assay for HbA1c estimation.
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